[Cloning and characterization of β-carbonic anhydrase, a potential drug target of

To express the beta carbonic anhydrase (β-CA) ofThe cDNA and amino sequence which may encode β-CA ofThe sequence Sjp_0056790.1 took on the conservative position of β-CAs. The PCR and restriction enzyme digestion confirmed the construction of recombinant plasmid pET-32a (+) -SjaCA. SDS-PAGE and Western blotting analyses showed that the molecular weight of recombinant protein was about 38 kDa as expected, and it could be recognized by anti-His tag antibody. The catalytic activity determining revealed that the recombinant protein SjaCA owned the carbonic anhydrase activity.Sjp_0056790.1 encodes the β-CA of［