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Neutralization of HSF1 in cells from PIK3CA-related overgrowth spectrum patients blocks abnormal proliferation
- Source :
- Biochemical and biophysical research communications. 530(3)
- Publication Year :
- 2020
-
Abstract
- PIK3CA-related overgrowth spectrum is caused by mosaicism mutations in the PIK3CA gene. These mutations, which are also observed in various types of cancer, lead to a constitutive activation of the PI3K/AKT/mTOR pathway, increasing cell proliferation. Heat shock transcription factor 1 (HSF1) is the major stress-responsive transcription factor. Recent findings indicate that AKT phosphorylates and activates HSF1 independently of heat-shock in breast cancer cells. Here, we aimed to investigate the role of HSF1 in PIK3CA-related overgrowth spectrum. We observed a higher rate of proliferation and increased phosphorylation of AKT and p70S6K in mutant fibroblasts than in control cells. We also found elevated phosphorylation and activation of HSF1, which is directly correlated to AKT activation. Specific AKT inhibitors inhibit HSF1 phosphorylation as well as HSF1-dependent gene transcription. Finally, we demonstrated that targeting HSF1 with specific inhibitors reduced the proliferation of mutant cells. As there is currently no curative treatment for PIK3CA-related overgrowth spectrum, our results identify HSF1 as a new potential therapeutic target.
- Subjects :
- Class I Phosphatidylinositol 3-Kinases
Vascular Malformations
Fibroblasts
Musculoskeletal Abnormalities
Heat Shock Transcription Factors
Drug Discovery
Mutation
Humans
Lipoma
Molecular Targeted Therapy
Phosphorylation
Nevus
Proto-Oncogene Proteins c-akt
Cells, Cultured
Cell Proliferation
Signal Transduction
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 530
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.pmid..........0f91bacb7686b81901ab2e5e92d6a71d