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4H12, a Murine Monoclonal Antibody Directed against Myosin Heavy Chain-9 Expressed on Acinar Cell Carcinoma of Pancreas with Potential Therapeutic Application
- Source :
- Iranian Biomedical Journal
- Publication Year :
- 2021
- Publisher :
- Pasteur Institute of Iran, 2021.
-
Abstract
- Pancreatic acinar cell carcinoma (PACC) is a rare type of pancreatic exocrine neoplasm that is frequently diagnosed at late stages with a high rate of metastasis. Identification of new biomarkers for PACC can improve our knowledge of its biology, early detection, or targeted therapy. In this study, hybridoma technology was used to generate mAbs against Faraz-ICR, a pancreatic acinar cell carcinoma cell line.Cell ELISA and flow cytometry were used for screening, and the 4H12 hybridoma clone was selected for further analysis. The 4H12 mAb was specific for myosin heavy chain-9 (MYH9) as determined by Immunoprecipitation, Western blot, and mass spectrometry.This antibody reacted variably with other cancer cells, in comparison to Faraz-ICR cell. Besides, by immunohistochemical staining, the acinar cell tumor, which was the source of Faraz-ICR, showed high MYH9 expression. Among 21 pancreatic ductal adenocarcinoma cases, nine (42.8%) expressed MYH9 with low intensity, while 10 (47.8%) and 2 (9.5%) cases expressed MYH9 with moderate to strong intensities, respectively. The 4H12 mAb inhibited the proliferation of Faraz-ICR cells in a dose-dependent manner from 0.75 to 12.5 μg/ml concentrations (p0.0001 and p0.002). IC50 values were achieved at 12.09 ± 4.19 µg/ml and 7.74 ± 4.28 µg/ml after 24- and 48-h treatment, respectively.Our data suggest that the 4H12 mAb can serve as a tool for investigating the role of MYH9 pancreatic cancer biology and prognosis.
- Subjects :
- Monoclonal antibody
Adult
Aged, 80 and over
Male
Mice, Inbred BALB C
Hybridomas
Myosin Heavy Chains
Carcinoma, Acinar Cell
Full Length
Antibodies, Monoclonal
Middle Aged
Pancreatic Neoplasms
Mice
Antigens, Neoplasm
Cell Line, Tumor
Animals
Humans
Female
Pancreas
Acinar cell carcinoma
Biomarkers
Aged
Cell Proliferation
Subjects
Details
- Language :
- English
- ISSN :
- 2008823X and 1028852X
- Volume :
- 25
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Iranian Biomedical Journal
- Accession number :
- edsair.pmid..........19222e3f86e64f31db82d254746faf81