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Induction of macrophage C-C chemokine expression by titanium alloy and bone cement particles

Authors :
Y, Nakashima
D H, Sun
M C, Trindade
L E, Chun
Y, Song
S B, Goodman
D J, Schurman
W J, Maloney
R L, Smith
Source :
The Journal of bone and joint surgery. British volume. 81(1)
Publication Year :
1999

Abstract

Particulate wear debris is associated with periprosthetic inflammation and loosening in total joint arthroplasty. We tested the effects of titanium alloy (Ti-alloy) and PMMA particles on monocyte/macrophage expression of the C-C chemokines, monocyte chemoattractant protein-1 (MCP-1), monocyte inflammatory protein-1 alpha (MIP-1alpha), and regulated upon activation normal T expressed and secreted protein (RANTES). Periprosthetic granulomatous tissue was analysed for expression of macrophage chemokines by immunohistochemistry. Chemokine expression in human monocytes/macrophages exposed to Ti-alloy and PMMA particles in vitro was determined by RT-PCR, ELISA and monocyte migration. We observed MCP-1 and MIP-1alpha expression in all tissue samples from failed arthroplasties. Ti-alloy and PMMA particles increased expression of MCP-1 and MIP-1alpha in macrophages in vitro in a dose- and time-dependent manner whereas RANTES was not detected. mRNA signal levels for MCP-1 and MIP-1alpha were also observed in cells after exposure to particles. Monocyte migration was stimulated by culture medium collected from macrophages exposed to Ti-alloy and PMMA particles. Antibodies to MCP-1 and MIP-1alpha inhibited chemotactic activity of the culture medium samples. Release of C-C chemokines by macrophages in response to wear particles may contribute to chronic inflammation at the bone-implant interface in total joint arthroplasty.

Details

ISSN :
0301620X
Volume :
81
Issue :
1
Database :
OpenAIRE
Journal :
The Journal of bone and joint surgery. British volume
Accession number :
edsair.pmid..........1ab3bcd0b4c95ad6716f227c7fb888ba