[Modification of chorionic carcinoma cells by immunomodulators. Cytokines and chorionic carcinoma cells]

The role of inflammatory cytokines on regulation of hCG biosynthesis was studied.JAR choriocarcinoma cells were cultured and treated with interleukin-1 (IL-1), IL-3. IL-4, stem cell factor (SCF), tumor necrosis factor (TNF), and granulocyte or granulocyte-macrophage-colony stimulating factor. hCG concentration was determined by immunoradiometric methods and cell number was enumerated by means of an electronic particle counter.Il-1 and TNF increased biosynthesis of holo-hCG as well as the free alpha- and free beta-subunit and the beta-core protein. Proliferation was not affected by any cytokine tested.Inflammatory cytokines are able to increase the biosynthesis of hCG.