[Preparation of cellulose-based chiral stationary phase and enantioseparation of ethoxyfen-ethyl by high performance liquid chromatography]

Cellulose-tris (3,5-dimethylphenylcarbamate) was prepared after a reported method, and was confirmed by infrared spectroscopy and elemental analysis. Then it was coated onto an amino-propylated mesopore spherical silica gel. The final product was used as the chiral stationary phase of high performance liquid chromatography for the enantioseparation of a novel herbicide ethoxyfen-ethyl. The enantioseparation of ethoxyfen-ethyl on this stationary phase has been achieved for the first time. Mixtures of hexane and isopropanol were used as mobile phases. The effects of isopropanol concentration in the mobile phase on the retention and resolution were investigated. With the decrease of the content of isopropanol in mobile phase, the resolution factors increased. When the isopropanol concentration decreased to one percent, the resolution factor was 3.95. The structural features of the solutes that influence chiral separation are also discussed.