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[Regulatory Effect of All-Trans Retinoic Acid on the Expression of IL-1β in Macrophages and the Mechanisms Involved]
- Source :
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition. 53(3)
- Publication Year :
- 2022
-
Abstract
- To investigate the regulatory effect of all-trans retinoic acid (ATRA) on the expression interleukin-1β (IL-1β) in macrophages and the mechanisms involved.Macrophages were treated with 1 μmol/L ATRA for 24 h before RNA-Sequence. Differentially expressed genes (DEGs) were screened out and analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, gene ontology (GO) functional analysis, and protein-protein interaction networks (PPI) analysis. After treatment with different doses of ATRA for 24 h, the expression of IL-1β was examined with qRT-PCR and Western blot. The activation of NF-κB signaling and caspase-1 was observed by Western blot and immunofluorescence staining.Compared with the blank control group, a total of 71 DEGs of macrophages were upregulated in the ATRA treatment group. KEGG analysis showed that the up-regulated DEGs were involved in IL-17 signaling pathway, tumor necrosis factor (TNF) signaling pathway, etc. GO analysis indicated that the up-regulated DEGs were involved in the biological processes of the production of IL-1β, response to lipopolysaccharide, etc. PPI analysis revealed that inflammatory cytokines, adhesion molecules, and chemokines were the key genes that ATRA acted on.ATRA may promote the expression of IL-1β by activating NF-κB signaling and caspase-1 in macrophages, this study may provide evidence for the immune regulatory function of ATRA on macrophages.
- Subjects :
- Macrophages
Caspase 1
Interleukin-1beta
NF-kappa B
Tretinoin
Subjects
Details
- ISSN :
- 1672173X
- Volume :
- 53
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
- Accession number :
- edsair.pmid..........27616a90aa61112a87905771b1a5415a