[Apoptosis of colon carcinoma cells of mice induced by vesicular stomatitis virus matrix protein in vitro]

To test the inhibitive effect of the matrix protein of vesicular stomatitis virus (VSV) on the proliferation of colon carcinoma cells of mice in vitro.The plasmid pcDNA3. 1-M encoding M protein of vesicular stomatitis viruses was transfected with lipofectamine 2000 into the colon carcinoma (CT26) cells of mice. The expression of VSV-M protein in the CT26 cells was detected by Western blot. The effect of vesicular stomatitis virus (VSV) matrix protein on the proliferation and apoptosis of colon carcinoma cells was measured by MTT, PI stainning and flow cytometry.The plasmid expressed M protein in the CT26 cells. Cytopathic effect was shown in the CT26 cells with transfected pcDNA3.1-M. The proliferation of the CT26 cells was suppressed significantly in vitro (76.4%, P0.05). The M protein induced apoptosis of the CT26 cells (80.2%), which was higher than'that of the controls (P0.05).The eukaryotic expression plasmid pcDNA3.1-M encoding M protein of vesicular stomatitis viruses induces apoptosis of CT26 cells, which has implications on the treatment of human colon cancer.