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In situ nuclear organization of regulatory machinery

In situ nuclear organization of regulatory machinery

Authors :
Shirwin M, Pockwinse
Sayyed K, Zaidi
Ricardo F, Medina
Rachit, Bakshi
Krishna P, Kota
Syed A, Ali
Daniel W, Young
Jeffery A, Nickerson
Amjad, Javed
Martin, Montecino
Andre J, van Wijnen
Jane B, Lian
Janet L, Stein
Gary S, Stein
Source :
Methods in molecular biology (Clifton, N.J.). 455
Publication Year :
2008

Abstract

Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.

Details

ISSN :
10643745
Volume :
455
Database :
OpenAIRE
Journal :
Methods in molecular biology (Clifton, N.J.)
Accession number :
edsair.pmid..........2c70c582d34bbaf1b48ea49f783af05a