[Gene Sequencing Analyses of 10 ABO Ambiguous Blood Group Samples]

Through analysis of ABO blood group gene typing technology, to assist in the identification of difficult clinical serological specimens.A total of 10 forwardreverse typing ambiguous samples were collected from January 2021 to August 2021 in our hospital.ABO genotypes were analysed by gene sequencing.The genotypes of 10 ABO ambiguous blood group samples were A102/BW11, A102/BW12, O02/O02, A102/B303, A102/B101, BW11/O02, B101/O04, BW11/O01, BW11/O01, A101/O02, respectively. The genotype results of 6 cases was consistent with the serological phenotype, and the serological phenotype of 4 cases were different from the geno sequencing.ABO blood groups genotyping technology combined with serological typing can be used for accurate typing of ambiguous blood group, and better ensure the safety of blood transfusion.10例ABO疑难血型的基因测序分析.利用ABO血型基因分型技术协助临床血清学疑难血型的鉴定.收集2021年1月－2021年8月本院用血清学鉴定ABO正反定型不符的疑难血型标本10例，采用基因测序方法检测ABO基因型.10例疑难血型标本的ABO基因型分别为A102/Bw11、A102/Bw12、O02/O02、A102/B303、A102/B101、Bw11/O02、B101/O04、Bw11/O01、Bw11/O01、A101/O02。其中有6例标本血型基因测序结果和血清学表型一致，其他4例标本的血清学表型和基因测序结果均表现出一定的差异.鉴定ABO疑难血型可利用基因分型技术结合血清学方法，最终准确鉴定血型，更好的保障输血安全.