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MAGI3 Suppresses Glioma Cell Proliferation via Upregulation of PTEN Expression
- Source :
- Biomedical and environmental sciences : BES. 28(7)
- Publication Year :
- 2014
-
Abstract
- To investigate the role and molecular mechanism of membrane-associated guanylate kinase inverted 3 (MAGI3) in glioma cell proliferation.The expression levels of MAGI3 and PTEN were assessed in glioma samples by Western blotting. MAGI3 was stably transfected into C6 glioma cells to obtain C6-MAGI3 cells. Then, the proliferation, the expression levels of MAGI3 and PTEN, and Akt phosphorylation were evaluated in C6 and C6-MAGI3 cells. Xenograft tumor models were established by subcutaneous injection of C6 and C6-MAGI3 cells into nude mice, and the growth rates of xenografts in the mice were compared. The potential role of MAGI3 expression in PI3K/Akt signaling activation was further investigated by examining the correlation between MAGI3 expression and the expression of PI3K/Akt signaling downstream target genes in a glioma dataset using gene set enrichment analysis (GSEA).Expression levels of MAGI3 and PTEN were significantly downregulated in gliomas. Overexpression of MAGI3 in the glioma C6 cell line upregulated PTEN protein expression, inhibited the phosphorylation of Akt, and suppressed cell proliferation. MAGI3 overexpression also inhibited the growth of C6 glioma tumor xenografts in nude mice. Analysis based on the GEO database confirmed the negative correlation between activation of PI3K/Akt pathway and MAGI3 mRNA levels in human glioma samples.The loss of MAGI3 expression in glioma may enhance the proliferation of glioma cells via downregulation of PTEN expression, leading to the activation of the PI3K/Akt pathway. MAGI3 is a potential glioma suppressor.
- Subjects :
- Brain Neoplasms
PTEN Phosphohydrolase
Down-Regulation
Membrane Proteins
Mice, Nude
Glioma
Transfection
Xenograft Model Antitumor Assays
Rats
Up-Regulation
Phosphatidylinositol 3-Kinases
Cell Line, Tumor
Animals
Humans
Phosphorylation
Proto-Oncogene Proteins c-akt
Cell Proliferation
Signal Transduction
Subjects
Details
- ISSN :
- 08953988
- Volume :
- 28
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Biomedical and environmental sciences : BES
- Accession number :
- edsair.pmid..........3c740a7d6986b411650d48f59c375002