Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c4ra01027b Click here for additional data file

We demonstrate super-resolution imaging of proteins and nucleic acids that were densely labelled with fluorophores using the concept of “click chemistry”.
We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins of E. coli.