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[Identification of genes induced to chondrocytes by nitric oxide]
- Source :
- Molekuliarnaia biologiia. 36(5)
- Publication Year :
- 2002
-
Abstract
- Nitric oxide (NO) acts as a short-lived paracrine factor and selectively activates transcription of certain genes. The spectrum of inducible genes was studied in primary chondrocytes. A cDNA library was obtained by subtraction hybridization with RNAs isolated from rabbit chondrocytes before and after treatment with nitrosoglutathione, an NO-generating agent. Some of the cloned cDNAs were homologous to known mammalian genes and human EST. NO-dependent transcriptional activation was demonstrated for the stromelysin 1 and cyclooxygenase 2 genes and, for the first time, for mcl1 coding for an apoptosis suppressor.
- Subjects :
- DNA, Complementary
Dose-Response Relationship, Drug
Nitric Oxide
Neoplasm Proteins
Rats
Isoenzymes
Chondrocytes
Gene Expression Regulation
Proto-Oncogene Proteins c-bcl-2
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
S-Nitrosoglutathione
Animals
Myeloid Cell Leukemia Sequence 1 Protein
Matrix Metalloproteinase 3
Nitric Oxide Donors
Rabbits
Rats, Wistar
Cells, Cultured
In Situ Hybridization
Gene Library
Subjects
Details
- ISSN :
- 00268984
- Volume :
- 36
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Molekuliarnaia biologiia
- Accession number :
- edsair.pmid..........430928f0f9ee4bd4c97ae2389300cf4e