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[Identification of genes induced to chondrocytes by nitric oxide]

Authors :
A I, Nikolaev
V I, Dubovaia
D Iu, Litvinov
A B, Poltaraus
D S, Ivanov
A M, Amchenkova
A N, NarovlianskiÄ­
A F, Panasiuk
V S, Prasolov
K T, Turpaev
Source :
Molekuliarnaia biologiia. 36(5)
Publication Year :
2002

Abstract

Nitric oxide (NO) acts as a short-lived paracrine factor and selectively activates transcription of certain genes. The spectrum of inducible genes was studied in primary chondrocytes. A cDNA library was obtained by subtraction hybridization with RNAs isolated from rabbit chondrocytes before and after treatment with nitrosoglutathione, an NO-generating agent. Some of the cloned cDNAs were homologous to known mammalian genes and human EST. NO-dependent transcriptional activation was demonstrated for the stromelysin 1 and cyclooxygenase 2 genes and, for the first time, for mcl1 coding for an apoptosis suppressor.

Details

ISSN :
00268984
Volume :
36
Issue :
5
Database :
OpenAIRE
Journal :
Molekuliarnaia biologiia
Accession number :
edsair.pmid..........430928f0f9ee4bd4c97ae2389300cf4e