[Effects of Soluble CD40 Ligand on Non-Hodgkin Lymphoma Cells and Its Relative Mechanism]

To explore the effect of soluble CD40 ligand (sCD40L) on the proliferation, apoptosis, and cell cycle of human non-Hodgkin lymphoma (NHL) cells, and analyze its possible mechanism.NHL CA46 cell and Raji cell were treated with different concentrations of sCD40L for 48 h, CCK-8 was used to detect the effect of sCD40L on cell proliferation in vitro, flow cytometry on apoptosis and cycle of NHL cells, and Western blot on the expression of PTEN, BCL-2, and BAX in NHL cells.Compared with the control group, 4 and 8 μg/ml sCD40L could significantly inhibit the proliferation of lymphoma Raji cell and CA46 cell (P0.05). The test results of flow cytometry showed that 4 μg/ml sCD40L could significantly promote the apoptosis of CA46 and Raji cells, and significantly inhibit the S phase proportions (P0.05). Western blot results showed that sCD40L could promote the expression of PTEN and BAX, while inhibit the expression of BCL-2 (P0.05).sCD40L can promote the apoptosis and inhibit the proliferation of NHL cells through the PTEN signaling pathway.可溶性CD40配体对非霍奇金淋巴瘤细胞的影响及相关机制.探讨可溶性CD40配体（sCD40L）对人非霍奇金淋巴瘤细胞增殖、凋亡及细胞周期的影响及其可能机制.用不同浓度的sCD40L处理非霍奇金淋巴瘤CA46细胞和Raji细胞48 h，用CCK-8检测细胞增殖；流式细胞术检测细胞凋亡和周期；通过蛋白免疫印迹检测PTEN、BCL-2和BAX的表达.与对照组比较，4和8 μg/ml的sCD40L均能显著抑制Raji细胞和CA46细胞的增殖（P0.05）。流式细胞术检测凋亡和细胞周期结果显示，4 μg/ml的sCD40L能显著促进CA46和Raji细胞的凋亡和减少S期细胞的比例（P0.05）；蛋白免疫印迹结果显示，sCD40L能显著促进PTEN和BAX的表达，抑制BCL-2的表达（P0.05）.sCD40L抑制淋巴瘤细胞的增殖并促进其凋亡可能是通过调控PTEN信号通路实现.