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Downregulation of gasdermin D promotes gastric cancer proliferation by regulating cell cycle-related proteins

Authors :
Wei Jie, Wang
Di, Chen
Ming Zuo, Jiang
Bing, Xu
Xiao Wei, Li
Yi, Chu
Yu Jie, Zhang
Ren, Mao
Jie, Liang
Dai Ming, Fan
Source :
Journal of digestive diseases. 19(2)
Publication Year :
2017

Abstract

To explore the relationship between gasdermin D (GSDMD) and gastric cancer (GC) cell proliferation, and to determine whether the downregulated expression of GSDMD contributed to the tumorigenesis and proliferation of GC cells.GSDMD expressions in GC tissues and matched adjacent non-cancerous tissues were assessed by quantitative real-time polymerase chain reaction, Western blot and immunohistochemistry. The effect of GSDMD on cell proliferation in vitro was assessed by the colony formation assay and cell viability assays. In vivo, xenografted tumors in nude mice were evaluated. The cell cycle was analyzed by flow cytometry. In addition, the alterations of several cell cycle-related and cell signaling pathway proteins were analyzed by Western blot.GSDMD expression was decreased in GC, and the decreased expression of GSDMD could markedly promote the proliferation of tumors in vivo and in vitro. The downregulation of GSDMD accelerated S/GGSDMD may protect against cell proliferation of GC, and it may be used as a diagnostic and treatment strategy for GC.

Details

ISSN :
17512980
Volume :
19
Issue :
2
Database :
OpenAIRE
Journal :
Journal of digestive diseases
Accession number :
edsair.pmid..........4d8bf59d928847039424f4ad906c6c9d