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Gene expression profiling of craniofacial fibrous dysplasia reveals ADAMTS2 overexpression as a potential marker
- Source :
- International journal of clinical and experimental pathology. 7(12)
- Publication Year :
- 2014
-
Abstract
- Fibrous dysplasia (FD) as an abnormal bone growth is one of the common fibro-osseous leasions (FOL) in oral and maxillofacial region, however, its etiology still remains unclear. Here, we performed gene expression profiling of FD using microarray analysis to explore the key molecule events in FD development, and develop potential diagnostic markers or therapeutic targets for FD. We found that 1,881 genes exhibited differential expression with more than two-fold changes in FD compared to normal bone tissues, including 1,200 upregulated genes and 681 downregulated genes. Pathway analysis indicated that obviously activated pathways are Ribosome and ECM-receptor interaction pathways; downregulated pathways are “Hepatitis C” and “cancer” signaling pathways. We further validated the expression of ADAMTS2, one of most differentiated expressed genes, by Immunohistochemistry (IHC) in 40 of FD cases. Results showed that ADAMTS2 was significantly overexpressed in FD tissues, but rarely expressed in normal bone tissues, suggesting that ADAMTS2 could be a potential biomarker for FD. Thus, this study uncovered differentially expressed candidate genes in FD, which provides pilot data for understanding FD pathogenesis, and developing novel biomarkers for diagnosis and targeting of FD.
- Subjects :
- Gene Expression Profiling
Skull
Fibrous Dysplasia, Polyostotic
Real-Time Polymerase Chain Reaction
Immunohistochemistry
Facial Bones
Up-Regulation
ADAM Proteins
ADAMTS Proteins
ADAMTS4 Protein
Humans
Original Article
Procollagen N-Endopeptidase
Transcriptome
Biomarkers
Oligonucleotide Array Sequence Analysis
Subjects
Details
- ISSN :
- 19362625
- Volume :
- 7
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- International journal of clinical and experimental pathology
- Accession number :
- edsair.pmid..........4e9f0f99d1f573295e387c8497e0e3ec