[Expression of alpha-SMA mRNA in the lung tissue of rat with asthma]

To investigate the expression of smooth muscle actin(SMA)-alpha mRNA in the lung of rat with asthma.A rat model of asthma was established by exposing the animal to aerosolized ovalbumin (OA). After being sensitized, the rat was subjected to laboratory tests. alpha-SMA mRNA of the lung tissue was measured by RT-PCR. The thickness of the airway smooth muscle(ASM) was measured by an image analysis system at the same time.The thickness of ASM increased 2 weeks after OA challenge. The expression of alpha-SMA mRNA increased 1 week after OA challenge and increased more significantly after 2 weeks.In the rat model of asthma, hypertrophy and hyperplasia of the airway smooth muscle were noted, and the phenotype was changed.