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[Study on optimization of SRAP-PCR reaction system for Pinellia ternata in Suzhou]
- Source :
- Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 37(24)
- Publication Year :
- 2013
-
Abstract
- To investigate the optimization system of SRAP-PCR molecular marker technology in the analysis on Pinellia ternata.SRAP-PCR reaction system for P. ternata was optimized by L16 (5(4)) orthogonal design with five elements (dNTPs, Mg2+, the template DNA, primers, Taq enzyme) and four standards.The most suitable forward primer for SRAP for Pinellia ternata was 5'-TGAGTCCAAACCGGAAG-3', while the reverse primer was 5'-GACTGCGTACGAATTACG-3'. The optimized reaction system contained 70 ng DNA template, 0.9 micromol x L(-1) primer, 0.20 mmol x L(-1) dNTP s, 1.5 - 2.0 mmol x L(-1) Mg2+, and 2 U Taq enzyme.SRAP-PCR system for P. ternata is established to lay a foundation for future construction of SRAP genetic map of P. ternata.
Details
- ISSN :
- 10015302
- Volume :
- 37
- Issue :
- 24
- Database :
- OpenAIRE
- Journal :
- Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
- Accession number :
- edsair.pmid..........538ddee8d3e09eb793752a650a40a88a