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MiR-210 is induced by Oct-2, regulates B-cells and inhibits autoantibody production1
- Publication Year :
- 2013
-
Abstract
- MicroRNAs (MiRs) are small, noncoding RNAs that regulate gene expression posttranscriptionally. In this study, we show that MiR-210 is induced by Oct-2, a key transcriptional mediator of B cell activation. Germline deletion of MiR-210 results in the development of autoantibodies from 5 mo of age. Overexpression of MiR-210 in vivo resulted in cell autonomous expansion of the B1 lineage and impaired fitness of B2 cells. Mice overexpressing MiR-210 exhibited impaired class-switched Ab responses, a finding confirmed in wild-type B cells transfected with a MiR-210 mimic. In vitro studies demonstrated defects in cellular proliferation and cell cycle entry, which were consistent with the transcriptomic analysis demonstrating downregulation of genes involved in cellular proliferation and B cell activation. These findings indicate that Oct-2 induction of MiR-210 provides a novel inhibitory mechanism for the control of B cells and autoantibody production.
- Subjects :
- B-Lymphocytes
Chromatin Immunoprecipitation
Fluorescent Antibody Technique
Enzyme-Linked Immunosorbent Assay
Mice, Transgenic
Cell Separation
Lymphocyte Activation
Polymerase Chain Reaction
Article
Mice, Inbred C57BL
Mice
MicroRNAs
Animals
Octamer Transcription Factor-2
Transcriptome
Autoantibodies
Oligonucleotide Array Sequence Analysis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.pmid..........5a6e2931aca074dd409bcf1f18b126a8