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Purification of PP2Ac from bovine heart
- Source :
- Methods in molecular biology (Clifton, N.J.). 365
- Publication Year :
- 2007
-
Abstract
- The catalytic subunit of PP2A (PP2Ac) can be purified in milligram quantities from bovine heart using ethanol precipitation, ammonium sulfate precipitation, ion exchange and size exclusion chromatography. The detailed procedure is described to purify PP2Ac over 4 d.
Details
- ISSN :
- 10643745
- Volume :
- 365
- Database :
- OpenAIRE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Accession number :
- edsair.pmid..........5b06e06f5c9a2f0181947fcdbf1539cd