Time-Dependent Toxicities of Quorum Sensing Inhibitors to

Quorum sensing inhibitors (QSIs) are being used widely as a promising alternative to antibiotics and drawing attention as potential pollutants. However, the assessment methods of the toxicities of QSIs, including model organism and affecting time, have not been established. To investigate how model organism and acting time impact the toxicities of QSIs, the effect of 4 QSIs to Aliivibrio fischeri and Bacillus subtilis were determined at different exposing time in the present study. The results showed that the toxic effects of QSIs to gram-negative bacteria (A fischeri) and gram-positive bacteria (B subtilis) were different and time dependent. As for A fischeri, QSI (furaneol acetate, FA) merely showed inhibition on the bioluminescence from hours 1 to 2. But from hours 3 to 6, low concentration FA exerted stimulation on the bioluminescence. Then, this stimulation disappeared from hours 7 to 14, and after hour 15 the stimulation appeared again. That is to say, QSIs showed intermittent hormesis effect on the bioluminescence of A fischeri. By contrast, only inhibition was observed in the toxicity test process of QSIs to B subtilis. As exposing time goes, the inhibition weakened gradually when FA was at low concentration regions. What is more, in the present, study toxic mechanisms were also discussed based on model organisms and exposing time. This study demonstrates appreciable impacts of model organism and exposing time on toxicities of QSIs and provides a theoretical basis for risk assessments after QSIs being widely used into the environment.