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Purification and characterization of two thermostable xylanases from a halotolerant Bacillus sp. Asc6BA isolated from Salar de Ascotán, Atacama Desert

Authors :
Francisca, Contreras
Maximiliano J, Amenabar
Jenny M, Blamey
Source :
Extremophiles : life under extreme conditions. 25(1)
Publication Year :
2020

Abstract

Two extracellular xylanases, denominated X2 and X3, were purified and characterized from the halotolerant bacterium Bacillus sp. Asc6BA isolated from "Salar de Ascotán" in the Atacama Desert. Xylanases were purified by anion exchange, cation exchange and size exclusion liquid chromatography. Xylanase X2 and X3 were purified ~ 690-fold and ~ 629-fold, respectively, compared to the concentrated extracellular fraction with a final specific activity of 169 and 154 u mg

Details

ISSN :
14334909
Volume :
25
Issue :
1
Database :
OpenAIRE
Journal :
Extremophiles : life under extreme conditions
Accession number :
edsair.pmid..........61c8fac2d592e877484cf32934b8a883