[Polarographic method for determining activity of dehydrogenases of the pentosephosphate pathway of carbohydrate metabolism]

A procedure is developed for estimation of glucose-6-phosphate and 6-phosphogluconate dehydrogenases activity in human erythrocytes. The procedure involved polarographic registration of oxygen consumption, which is directly related to content of NADPH formed, in a coupled enzymatic system containing peroxidase and Mn2+.