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Gradual upregulation of OCI-5 expression during occurrence and progression of rat hepatocellular carcinoma

Authors :
Wei-Jun, Jiang
Xiao-Bo, Man
Liang, Tang
Hong-Yin, Song
Shen-Jing, Li
Guo-Jun, Cai
Xiu-Hua, Qiu
He-Ping, Hu
Source :
Hepatobiliarypancreatic diseases international : HBPD INT. 5(2)
Publication Year :
2006

Abstract

OCI-5, the rat homologene of human glypican 3 (GPC3), is confirmed upregulated in hepatocellular carcinoma (HCC). The present study was undertaken to detect gene expression change of OCI-5 during occurrence and progression of rat HCC.Male Sprague-Dawley rats were given diethylnitrosamine (DENA) to induce HCC. Three DENA-induced rats and one control rat were sacrificed every week for 18 weeks during the development of HCC. Tissues specimens were snap-frozen in liquid nitrogen and total RNA was isolated. Sk-Hep1 cells were treated with DENA at different concentrations. The gene expression levels of OCI-5 and GPC3 were detected with the RT-PCR method.OCI-5 was not expressed in normal rat liver tissues. When HCC occurred and aggravated, OCI-5 expression was gradually elevated to a very high level. GPC3 was not expressed in the DENA-treated Sk-Hep1 cells.OCI-5 was not expressed in normal rat liver tissues but in rat HCC tissues. High-expression of OCI-5 in DENA-induced rat HCC model was the gene expression change of HCC not the DENA-induced gene expression. The expression level of OCI-5 was not only elevated in rat HCC but also gradually along the occurrence and progression of HCC, indicating that GPC3 might serve as a sensitive marker of early stage HCC.

Details

ISSN :
14993872
Volume :
5
Issue :
2
Database :
OpenAIRE
Journal :
Hepatobiliarypancreatic diseases international : HBPD INT
Accession number :
edsair.pmid..........6742d1a8d8f66cd1443e311cd6469585