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The LuxR family protein SpnR functions as a negative regulator of N-acylhomoserine lactone-dependent quorum sensing in Serratia marcescens
- Source :
- Molecular microbiology. 45(6)
- Publication Year :
- 2002
-
Abstract
- Serratia marcescens SS-1 produces at least four N-acylhomoserine lactones (AHLs) which were identified using high-resolution mass spectrometry and chemical synthesis, as N-(3-oxohexanoyl) homo-serine lactone (3-oxo-C6-HSL), N-hexanoyl- (C6-HSL), N-heptanoyl (C7-HSL) and N-octanoyl- (C8-HSL) homoserine lactone. These AHLs are synthesized via the LuxI homologue SpnI, and regulate via the LuxR homologue SpnR, the production of the red pigment, prodigiosin, the nuclease, NucA, and a biosurfactant which facilitates surface translocation. spnR overexpression and spnR gene deletion show that SpnR, in contrast to most LuxR homologues, acts as a negative regulator. spnI overexpression, the provision of exogenous AHLs and spnI gene deletion suggest that SpnR is de-repressed by 3-oxo-C6-HSL. In addition, long chain AHLs antagonize the biosurfactant-mediated surface translocation of S. marcescens SS-1. Upstream of spnI there is a gene which we have termed spnT. spnI and spnT form an operon and although database searches failed to reveal any spnT homologues, overexpression of this novel gene negatively affected both sliding motility and prodigiosin production.
- Subjects :
- Deoxyribonucleases
Base Sequence
Movement
Prodigiosin
Molecular Sequence Data
RNA-Binding Proteins
Gene Expression Regulation, Bacterial
Repressor Proteins
4-Butyrolactone
Bacterial Proteins
Flagella
Trans-Activators
Amino Acid Sequence
Microtubule-Associated Proteins
Serratia marcescens
Signal Transduction
Subjects
Details
- ISSN :
- 0950382X
- Volume :
- 45
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecular microbiology
- Accession number :
- edsair.pmid..........692deea1df0ca4be02975d7ff618f239