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Single-Cell Sequencing and Organoids: A Powerful Combination for Modelling Organ Development and Diseases

Authors :
Yuebang, Yin
Peng-Yu, Liu
Yinghua, Shi
Ping, Li
Source :
Reviews of physiology, biochemistry and pharmacology. 179
Publication Year :
2021

Abstract

The development and function of a particular organ and the pathogenesis of various diseases remain intimately linked to the features of each cell type in the organ. Conventional messenger RNA- or protein-based methodologies often fail to elucidate the contribution of rare cell types, including some subpopulations of stem cells, short-lived progenitors and circulating tumour cells, thus hampering their applications in studies regarding organ development and diseases. The scRNA-seq technique represents a new approach for determining gene expression variability at the single-cell level. Organoids are new preclinical models that recapitulate complete or partial features of their original organ and are thought to be superior to cell models in mimicking the sophisticated spatiotemporal processes of the development and regeneration and diseases. In this review, we highlight recent advances in the field of scRNA-seq, organoids and their current applications and summarize the advantages of using a combination of scRNA-seq and organoid technology to model diseases and organ development.

Subjects

Subjects :
Organoids
Stem Cells
RNA, Messenger

Details

ISSN :
03034240
Volume :
179
Database :
OpenAIRE
Journal :
Reviews of physiology, biochemistry and pharmacology
Accession number :
edsair.pmid..........787abf7f4f6271ef8c37e20e538a7f9c