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Corilagin potential in inhibiting oxidative and inflammatory stress in LPS-induced murine macrophage cell lines (RAW 264.7)
- Source :
- Iran J Basic Med Sci
- Publication Year :
- 2021
- Publisher :
- Mashhad University of Medical Sciences, 2021.
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Abstract
- OBJECTIVE(S): Inflammation is thought to be the common pathophysiological basis for several disorders. Corilagin is one of the major active compounds which showed broad-spectrum biological and therapeutic activities, such as antitumor, hepatoprotective, anti-oxidant, and anti-inflammatory. This study aimed to evaluate the anti-oxidant and anti-inflammatory activities of corilagin in LPS-induced RAW264.7 cells. MATERIALS AND METHODS: Anti-oxidant activities were examined by free radical scavenging of H(2)O(2), NO, and *OH. The safe concentrations of corilagin on RAW264.7 were determined by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay on RAW264.7 cell lines. The inflammation cells model was induced with LPS. The anti-inflammatory activities measured IL-6, TNF-α, NO, IL-1β, PGE-2, iNOS, and COX-2 levels using ELISA assay. RESULTS: The results showed that corilagin had a significant inhibition activity dose-dependently in scavenging activities toward H(2)O(2), *OH, and NO with IC(50) values 76.85 µg/ml, 26.68 µg/ml, and 66.64 µg/ml, respectively. The anti-inflammatory activity of corilagin also showed a significant decrease toward IL-6, TNF-α, NO, IL-1β, PGE-2, iNOS, and COX-2 levels at the highest concentration (75 µM) compared with others concentration (50 and 25 µM) with the highest inhibition activities being 48.09%, 42.37%, 65.69%, 26.47%, 46.88%, 56.22%, 59.99%, respectively (P
- Subjects :
- Original Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Iran J Basic Med Sci
- Accession number :
- edsair.pmid..........7d02c21fece9b2153b7c504feef2949d