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Miniature structured illumination microscope for

Authors :
Omkar D, Supekar
Andrew, Sias
Sean R, Hansen
Gabriel, Martinez
Graham C, Peet
Xiaoyu, Peng
Victor M, Bright
Ethan G, Hughes
Diego, Restrepo
Douglas P, Shepherd
Cristin G, Welle
Juliet T, Gopinath
Emily A, Gibson
Source :
Biomed Opt Express
Publication Year :
2021

Abstract

We present a high-resolution miniature, light-weight fluorescence microscope with electrowetting lens and onboard CMOS for high resolution volumetric imaging and structured illumination for rejection of out-of-focus and scattered light. The miniature microscope (SIMscope3D) delivers structured light using a coherent fiber bundle to obtain optical sectioning with an axial resolution of 18 µm. Volumetric imaging of eGFP labeled cells in fixed mouse brain tissue at depths up to 260 µm is demonstrated. The functionality of SIMscope3D to provide background free 3D imaging is shown by recording time series of microglia dynamics in awake mice at depths up to 120 µm in the brain.

Subjects

Subjects :
Article

Details

ISSN :
21567085
Volume :
13
Issue :
4
Database :
OpenAIRE
Journal :
Biomedical optics express
Accession number :
edsair.pmid..........7e0c5a54ab568d554e12de1391566fb9