[Factors Affecting the Preservation of Human Peripheral Blood Mononuclear Cells at 4 ℃]

To analyze the preservation effect and related influencing factors of human peripheral blood mononuclear cells under serum-free condition at 4 ℃.Human peripheral blood mononuclear cells were isolated by density gradient centrifugation, and stored at 4 ℃ under different cell concentrations, supplemented with human serum albumin, and glucose. The cell viability, total cell number, viable cell number and cell phenotype were detected during preservation of 72 h.With the prolongation of storage time, the number of human peripheral blood mononuclear cells gradually decreased(The cell density of (1-2)×10影响人外周血单个核细胞4 ℃保存效果的因素分析.分析4 ℃无血清条件下人外周血单个核细胞保存效果及其相关影响因素.采用密度梯度离心法分离人外周血单个核细胞，使用不同细胞密度、添加人血清白蛋白、葡萄糖等条件对其进行4 ℃保存，并检测保存72 h内细胞的存活率、细胞数量、活细胞数量和细胞表型.随保存时间延长，人外周血单个核细胞数量逐步下降((1-2)×10