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Regulation of the biogenesis of OXPHOS complexes in cell transition from replicating to quiescent state: involvement of PKA and effect of hydroxytyrosol
- Source :
- Biochimica et biophysica acta. 1843(4)
- Publication Year :
- 2013
-
Abstract
- A study is presented on the expression of mitochondrial oxidative phosphorylation complexes in exponentially growing and serum-starved, quiescent human fibroblast cultures. The functional levels of respiratory complexes I and III and complex V (adenosine triphosphate (ATP) synthase) were found to be severely depressed in serum-starved fibroblasts. The depression of oxidative phosphorylation system (OXPHOS) complexes was associated with reduced levels of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and the down-stream nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factors (TFAM). In serum-starved fibroblasts decrease of the catalytic activity of AMP cyclic dependent protein kinase (PKA) and phosphorylation of cAMP response element-binding protein (CREB), the transcription coactivator of the PGC-1α gene, was found. Hydroxytyrosol prevented the decline in the expression of the PGC-1α transcription cascade of OXPHOS complexes in serum-starved fibroblast cultures. The positive effect of HT was associated with activation of PKA and CREB phosphorylation. These results show involvement of PKA, CREB and PGC-1α in the regulation of OXPHOS in cell transition from the replicating to the quiescent state.
- Subjects :
- Electron Transport Complex I
Nuclear Respiratory Factor 1
Peroxisome Proliferator-Activated Receptors
Hydrogen Peroxide
Fibroblasts
Phenylethyl Alcohol
Cyclic AMP-Dependent Protein Kinases
Oxidative Phosphorylation
Mitochondria
ATP Synthetase Complexes
Adenosine Triphosphate
Humans
Cyclic AMP Response Element-Binding Protein
Signal Transduction
Subjects
Details
- ISSN :
- 00063002
- Volume :
- 1843
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biochimica et biophysica acta
- Accession number :
- edsair.pmid..........87f2518e397cb97fcc371f985930f95b