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Development of a novel and specialized cultivation method for isolating Helicobacter pullorum from chicken meat
- Source :
- Iran J Vet Res
- Publication Year :
- 2021
- Publisher :
- School of Veterinary Medicine, University of Shiraz, 2021.
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Abstract
- BACKGROUND: It has become established that Helicobacter pullorum could be isolated from raw chicken meat. AIMS: This study was aimed to develop a novel culture method (protocol B) to isolate H. pullorum from chicken meat by adding some modifications to the traditional culture method (protocol A), and as a consequence to compare their sensitivity, specificity, and the accuracy of these methods with polymerase chain reaction (PCR) test. METHODS: 400 chicken meat samples were collected from various retail markets and supermarkets. Each sample was processed by protocol A, protocol B, and PCR test. RESULTS: Out of 400 samples, 77 (19.25%), and 163 (40.75%) were culture-positive by protocol A and protocol B, respectively. Using PCR test as a gold standard, 196 (49%) samples were identified as H. pullorum. The specificity for both protocols was determined 100%, while the sensitivity of protocol B and protocol A was assessed 83% and 39%, respectively. Also, the higher and lower accuracy belonged to protocol B (92%) and protocol A (70%), respectively. CONCLUSION: The methodology designed herein can provide a suitable, approximately sensitive, specific, and accurate method to cultivate H. pullorum from chicken meat.
- Subjects :
- food and beverages
Short Paper
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Iran J Vet Res
- Accession number :
- edsair.pmid..........89d7ef6f1836ee6c33eb565d87071b8a