Cyanobacterial Genome Sequencing, Annotation, and Bioinformatics

Cyanobacteria are collectively a globally important monophyletic phylum of bacteria. They have attracted a lot of attention, not only because they are rich sources of natural bioactive products, including toxic substances, but also because they play an important role in global nitrogen and carbon cycles, and are capable of maintaining versatile environmental niche adaptations. A vast number of cyanobacterial genomes have become available due to fast development of sequencing technologies, but effort is still needed to comprehensively understand the molecular basis of their diversity. Here, we introduce a basic pipeline for the cyanobacterial genome sequencing project that can be employed to complete the whole cyanobacterial genome. The pipeline includes DNA extraction from the cyanobacterial culture of interest, hybrid genome sequencing, and genome assembly and annotation. At the end of the chapter, we briefly introduce genome mining tools and one successful genome mining example from our laboratory. This chapter provides general guidance regarding the sequencing project and thus includes several references for alternative methods and tools so that the reader can easily modify the pipeline according to the needs of the laboratory.