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Validation of Appropriate Reference Genes for qRT-PCR Normalization in Oat (
- Source :
- International journal of molecular sciences. 23(19)
- Publication Year :
- 2022
-
Abstract
- Oat is a food and forage crop species widely cultivated worldwide, and it is also an important forage grass in plateau regions of China, where there is a high level of ultraviolet radiation and sunlight. Screening suitable reference genes for oat under UV-B and high-light stresses is a prerequisite for ensuring the accuracy of real-time quantitative PCR (qRT-PCR) data used in plant adaptation research. In this study, eight candidate reference genes (sulfite oxidase
- Subjects :
- Avena
Ultraviolet Rays
Gene Expression Profiling
Glyceraldehyde-3-Phosphate Dehydrogenases
Reproducibility of Results
Reference Standards
Real-Time Polymerase Chain Reaction
TATA-Box Binding Protein
Actins
Peptide Elongation Factor 1
Gene Expression Regulation, Plant
Ubiquitin-Conjugating Enzymes
Oxidoreductases Acting on Sulfur Group Donors
Phenylalanine Ammonia-Lyase
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 23
- Issue :
- 19
- Database :
- OpenAIRE
- Journal :
- International journal of molecular sciences
- Accession number :
- edsair.pmid..........a145d219cc95b548da6135ee092112a7