[Effect of miR-100 on Migration of Rat Bone Marrow Mesenchymal Stem Cells]

To explore the effect of miR-100 on the migration of rat bone marrow mesenchymal stem cells(rBMMSC).The rBMMSC were isolated by density gradient centrifugation, cell surface epitopes of CD105, CD45, CD34, CD29 and CD44 were analyzed by flow cytometry. The rBMMSC were transfected with miR-100 mimic or inhibitor, then the expression of miR-100 in transfected cells was detected by real-time PCR. Migration test was used to observe the effect of miR-100 on cell migration ability. The secretion level of chemokine SDF-1 in culture supernatant of cells was quantitatively detected by ELISA.The isolated cells were identified as BMMSC. After rBMMSC were transfected with miR-100 mimic or inhibitor, as compared with control group,the expression of miR-100 in rBMMSC significantly increased or decreased respectively. In the migration experiment, the rBMMSC migration was significantly inhibited in the miR-100 mimic group (P0.01), while the rBMMSC migration was significantly enhanced in the miR-100 inhibitor group (P0.01). The concentration of SDF-1 in the supernatant of the miR-100 mimic group and the miR-100 inhibitor group did not change significantly compared with the control group (P0.05).miR-100 can significantly inhibit the migration of rBMMSC, but not significantly correlated with the SDF-1.miR-100对大鼠骨髓间充质干细胞迁移能力的影响.探讨MicroRNA-100（miR-100）对大鼠骨髓间充质干细胞（rBMMSC）迁移能力的影响.分离培养rBMMSC，采用流式细胞术检测rBMMSC表面标志物CD105、CD45、CD34、CD29和CD44的表达。用miR-100类似物或抑制剂转染rBMMSC，应用实时定量PCR检测转染后细胞miR-100表达量。迁移实验测定转染后细胞迁移能力，观察miR-100对细胞迁移能力的影响。ELISA法定量检测培养上清中趋化因子SDF-1的分泌水平.经鉴定所分离的细胞确定为rBMMSC。用miR-100类似物或抑制剂转染rBMMSC后，与对照组相比，转染miR-100类似物的细胞miR-100表达显著上调(P0.01)，转染miR-100抑制剂的细胞miR-100表达显著下调(P0.01)。在迁移实验中，转染miR-100类似物可显著抑制rBMMSC迁移(P0.01)，而转染miR-100抑制剂可显著增强rBMMSC迁移(P0.01)。转染miR-100类似物组、转染miR-100抑制剂组的培养上清中，SDF-1浓度与对照组相比未见明显变化（P＞0.05）.miR-100可显著抑制rBMMSC的迁移，但与趋化因子SDF-1无明显关系.