[Construction of Runx1 transgenic mice]

Authors :: Xiaoju, Tang
Junli, Li
Xiaojing, Liu
Yi, Lei
Qiaoli, Su
Qingbo, Wu
Fengming, Luo
Source :: Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi. 30(3)
Publication Year :: 2013
Abstract: This study was aimed to construct transgenic mouse model with target for Runxl gene. Runxl cDNA of mice was amplified by PCR from pcDNA3. 1 Flag Runx1 FL vector and inserted into ptetO7-Asc-IRES-EGFP vector to form a recombinant vector, and then the recombinant vector was injected into fertilized egg by microinjection technology to get a transgenic mouse. The results of PCR and Southern blot indicated that the Runx1 transgenic mouse was constructed successfully, and this could provide an important tool for studying the function of Runxl gene in vivo.

Subjects :: Male
Mice, Inbred C57BL
Recombination, Genetic
Mice
Base Sequence
Microinjections
Core Binding Factor Alpha 2 Subunit
Genetic Vectors
Molecular Sequence Data
Animals
Female
Mice, Transgenic

ISSN :: 10015515
Volume :: 30
Issue :: 3
Database :: OpenAIRE
Journal :: Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Accession number :: edsair.pmid..........a4133e7dd7d2de6cf466f8e9b2fa7d8c

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