[HPLC fingerprint of flavonoids in fruits of Polygonum orientale]

To compare HPLC fingerprint of flavonoids in fruits of Polygonnm orientale from different regions.Diamonsil C18 (250 mm x 4.6 mm, 5 microm) had been used, the mobile phase consisted of methanol and 0.2% phosphoric acid as fradient eluent. The flow rate was 1 ml/min, and the detection wavelength was 310 nm.The finerprint of flaxonoids in fruits of Poligonum orientale with 11 common peaks was eatablished. The realative retention time and the range of relative area of the common peaks were determined.The established fingerprint can be used for the identification and quality control of flavonoids of Poligonum orientale from different regions.