Back to Search
Start Over
Undernutrition impairs the quality of growth plate and trabecular and cortical bones in growing rats 1
- Source :
- Acta Cirúrgica Brasileira
- Publication Year :
- 2019
- Publisher :
- Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2019.
-
Abstract
- Purpose To investigate the effects of dietary restriction on the growth plate and long bone tissue in growing rats. Methods Sixty male Wistar rats were randomly assigned to two groups: Control (Con) and Diet-restricted (Res). After weaning, the Res rats were offered 50% of the chow ingested by the control (ad libitum food intake). The animals were subdivided into two subgroups with follow-ups up to 56 or 70 days. After euthanasia, the growth plate of tibias was analyzed by histomorphometry, micro-computed tomography, and mechanical test. The trabecular and compact bones were evaluated by histomorphometry, dual-energy X-ray absorptiometry, and micro-computed tomography (μCT). Real-time PCR was used to analyze gene expression. Results Although dietary restriction did not alter gene expression, several phenotypic changes were seen in the growth plate; i.e., decrease in volume, reduction in total area and height, decrease in the area ossified zones, mechanical weakening, reduction in mass of trabecular and cortical bone, lower bone density, deterioration of the trabecular and cortical microarchitecture, and trabeculae with lower collagen deposition. Conclusion Dietary restriction had severe detrimental effects on the growth plate and trabecular and cortical bone.
Details
- Language :
- English
- ISSN :
- 16782674 and 01028650
- Volume :
- 34
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Acta Cirúrgica Brasileira
- Accession number :
- edsair.pmid..........a8445e8166c4be5048295b39024acef7