[Gene clone and sequence analysis of retinal specific rho promoter]

To obtain the gene encoding retinal specific Rho promoter therefore to prepare for exogenous gene transcription and expression in retina.Extract the genomic DNA of a BLAB/c mice . The Rho promoter DNA were then amplified by PCR, the product of which was subcloned into PcDNA3.1 +vector. After identification by restriction enzymes, the recombinant PcDNA3.1+vectors were subjected to sequence analysis.The fragments of the promoter as amplified by PCR were of predicted length. Digestion with HindIII and NheI proved correct insertion of the target fragments with expected length into the recombinant PcDNA3.1+vectors. As indicated by homology analysis the Rho promoter was highly conservative, and the Rho promoter we got had hole homology as reported by Boulanger A etc.Retinal specific Rho promoter has been successfully cloned, thus making possible the subsequent research as the mechanism and gene therapy of retina.