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1H-NMR spectroscopy of the glycoprotein-bound large carbohydrates from embryonal carcinoma cells
- Source :
- Biochemistry international. 9(5)
- Publication Year :
- 1984
-
Abstract
- 400 MHz NMR spectrum was recorded for the glycoprotein -bound large carbohydrates (embryoglycan) isolated from F9 embryonal carcinoma cells. Two intense signals at 4.13 ppm and 4.69 ppm were assigned to be H-4 of galactosyl residues substituted at C-3 and H-1 of G1cNAc beta 1----3, respectively. The result is consistent with the proposal that the fundamental building unit of the large glycan is G1cNAc beta 1----3Ga1 beta. Furthermore, the spectral data confirmed a conclusion obtained by glycosidase digestion that fucosyl residues are linked mostly to N-acetylglucosamine rather than galactose.
Details
- ISSN :
- 01585231
- Volume :
- 9
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Biochemistry international
- Accession number :
- edsair.pmid..........aef357607c076b5bb85efc4c38e39960