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Oligopeptide uptake and aminoglycoside resistance in Escherichia coli K12

Authors :
Eduardo H, Nakamatsu
Erica, Fujihira
Rita C C, Ferreira
Andréa, Balan
Sérgio O P, Costa
Luís C S, Ferreira
Source :
FEMS microbiology letters. 269(2)
Publication Year :
2007

Abstract

Previous reports have suggested that Escherichia coli K12 mutants defective in the expression of oligogopeptide permease protein A (OppA) exhibit reduced sensitivity to aminoglycosides due to altered permeability of the cell envelope. In this work, the role of the OppA protein, and the oligogopeptide permease (Opp) transport system has been evaluated, in the resistance to aminoglycosides using derivatives of the E. coli K12 SS320 strain selected for triornithine resistance or with a deletion of the complete opp operon. All tested mutants were defective in the uptake of tri- and tetra-peptides but did not expressed resistance to aminoglycosides. Additionally, complementation tests carried out with a plasmid encoding the OppA protein did not affect the sensitivity of the strains to these antibiotics. Taken together, these evidences indicate that the Opp uptake system, as well as the OppA protein, does not play a direct role in the sensitivity to aminoglycosides in E. coli K12.

Details

ISSN :
03781097
Volume :
269
Issue :
2
Database :
OpenAIRE
Journal :
FEMS microbiology letters
Accession number :
edsair.pmid..........af09cc4a811eb48cfaeaeb870440ea7f