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- Source :
- Aging (Albany NY)
- Publication Year :
- 2019
-
Abstract
- Renal fibrosis is a key factor in chronic kidney disease (CKD). Long non-coding RNAs (lncRNAs) play important roles in the physiological and pathological progression of human diseases. However, the roles and underlying mechanisms of lncRNAs in renal fibrosis still need to be discovered. In this study, we first displayed the increased lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) expression in renal fibrosis in patients with obstructive nephropathy (ON). Then we found that transforming growth factor beta 1 (TGF-β1) induced epithelial-mesenchymal transition (EMT) and extracellular matrix (ECM) protein deposition, which promoted the viability, proliferation and migration of human renal proximal tubular epithelial (HK2) cells. Next, MALAT1/miR-145/focal adhesion kinase (FAK) pathway was confirmed to play an importment role in TGF-β1-induced renal fibrosis. In addition, the MALAT1/miR-145/FAK pathway was involved in the effect of dihydroartemisinin (DHA) on TGF-β1-induced renal fibrosis in vitro and in vivo. Furthermore, m6A methyltransferase methyltransferase-like 3 (METTL3) was shown to be the main methyltransferase of m6A modification on MALAT1.
- Subjects :
- Male
Adenosine
Epithelial-Mesenchymal Transition
Down-Regulation
Methyltransferases
Kidney
Fibrosis
renal fibrosis
Transforming Growth Factor beta1
Mice
MicroRNAs
dihydroartemisinin
Focal Adhesion Protein-Tyrosine Kinases
TGF-β1
mA 6
Animals
Humans
Kidney Diseases
RNA, Long Noncoding
MALAT1
Signal Transduction
Research Paper
Subjects
Details
- ISSN :
- 19454589
- Volume :
- 12
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Aging
- Accession number :
- edsair.pmid..........b3c368d2867696727989700ce1fcacae