Back to Search
Start Over
Increased expression of Foxp3 in splenic CD8+ T cells from mice with anterior chamber-associated immune deviation
- Source :
- Molecular Vision
- Publication Year :
- 2007
- Publisher :
- Molecular Vision, 2007.
-
Abstract
- Purpose To examine the expression of Foxp3 on CD8+ T cells in the spleen during anterior chamber-associated immune deviation (ACAID). Methods Ovalbumin (OVA) was injected into the anterior chamber (AC) of C57BL/6 mice, and the delayed-type hypersensitivity (DTH) response was measured to evaluate the development of ACAID. The suppressive effect of CD8+ T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. Flow cytometry was used to assay the frequency of CD8+Foxp3+ T cells from normal mice, ACAID mice, and control mice receiving an AC injection of PBS (PBS-AC-injected mice). These frequencies were also tested after polyclonal or specific antigen stimulation. The mRNA level of Foxp3 in CD8+ splenocytes from different groups with or without stimulation were determined by reverse transcription-polymerase chain reaction. Results OVA injection into the AC induced ACAID, and CD8+ T cells from ACAID mice inhibited the ear-swelling response by OVA-primed responder cells in LAT assay. Flow cytometry analysis showed that the frequency of CD8+Foxp3+ cells in splenocytes was upregulated in ACAID mice following polyclonal or specific antigen stimulation. Foxp3 mRNA was only detected in CD8+ T cells from ACAID mice after polyclonal stimulation. Conclusions An upregulated Foxp3 expression in CD8+ T cells is associated with the development of ACAID, suggesting an involvement of CD8+Foxp3+ T cells in this model of immune tolerance.
- Subjects :
- Anterior Chamber
Ovalbumin
Reverse Transcriptase Polymerase Chain Reaction
chemical and pharmacologic phenomena
Forkhead Transcription Factors
CD8-Positive T-Lymphocytes
Flow Cytometry
Lymphocyte Activation
Adoptive Transfer
Up-Regulation
Mice, Inbred C57BL
Mice
Immune Tolerance
Animals
Edema
Hypersensitivity, Delayed
Lymphocyte Count
RNA, Messenger
Antigens
Ear Diseases
Cells, Cultured
Spleen
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 10900535
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Molecular Vision
- Accession number :
- edsair.pmid..........bbfabc3b697d9879c1ea9cb76708f734