Back to Search
Start Over
Three-Dimensional Genome Interactions Identify Potential Adipocyte Metabolism-Associated Gene
- Source :
- Frontiers in Endocrinology
- Publication Year :
- 2021
-
Abstract
- Background rs13405728 was identified as one of the most prevalent susceptibility loci for polycystic ovary syndrome (PCOS) in Han Chinese and Caucasian women. However, the target genes and potential mechanisms of the rs13405728 locus remain to be determined. Methods Three-dimensional (3D) genome interactions from the ovary tissue were characterized via high-through chromosome conformation capture (Hi-C) and Capture Hi-C technologies to identify putative targets at the rs13405728 locus. Combined analyses of eQTL, RNA-Seq, DNase-Seq, ChIP-Seq, and sing-cell sequencing were performed to explore the molecular roles of these target genes in PCOS. PCOS-like mice were applied to verify the expression patterns. Results Generally, STON1 and FSHR were identified as potential targets of the rs13405728 locus in 3D genomic interactions with epigenomic regulatory peaks, with STON1 (P=0.0423) and FSHR (P=0.0013) being highly expressed in PCOS patients. STON1 co-expressed genes were associated with metabolic processes (P=0.0008) in adipocytes (P=0.0001), which was validated in the fat tissue (P
- Subjects :
- endocrine system diseases
Gene Expression
three-dimensional genome analysis
Endocrinology
FSHR
Adipocytes
PCOS
Animals
Humans
Original Research
Genome
Ovary
Membrane Proteins
female genital diseases and pregnancy complications
rs13405728 locus
STON1
Mice, Inbred C57BL
Adipose Tissue
Genetic Loci
Receptors, Androgen
CD4 Antigens
Receptors, FSH
Female
Transcription Factors, General
Polycystic Ovary Syndrome
AR
Subjects
Details
- ISSN :
- 16642392
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in endocrinology
- Accession number :
- edsair.pmid..........c67c5d4ec8a619d1e70462ce53459b4a