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Hsa_circ_0102533 serves as a blood-based biomarker for non-small-cell lung cancer diagnosis and regulates apoptosis in vitro
- Publication Year :
- 2018
- Publisher :
- e-Century Publishing Corporation, 2018.
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Abstract
- Background: Circular RNAs (circRNAs) as a new class of non-coding RNAs that are associated with cancer progression and can serve as potential markers for cancer diagnosis. However, the functions of circRNAs have not been completely clarified in non-small-cell lung cancer (NSCLC). Our study aimed to explore the expression profiles and apoptotic role of circRNAs in NSCLC. Methods: Forty-one NSCLC patients and twenty-six healthy subjects were recruited to our study. The levels of hsa_circ_0102533 in tumor tissues and whole blood were identified by circRNA microarray and RT-qPCR. The CCK-8 and apoptosis assays were performed in NSCLC cell lines after they were transfected with si-circRNA and si-NC. Results: Compared with the control group, hsa_circ_0102533 expression was significantly increased in tumor tissues and whole blood from NSCLC patients. ROC analysis showed that hsa_circ_0102533 had a higher diagnostic power for the detection of cancer in stage I-II NSCLC patients [AUC: 0.774 (95% CI: 0.624-0.923)] than in stage III-IV NSCLC patients [0.728 (95% CI: 0.588-0.869)]. Furthermore, the knockout of hsa_circ_0102533 by siRNA significantly inhibited proliferation and induced apoptosis in NSCLC cell lines. Conclusion: Hsa_circ_0102533 possesses an oncogenic property in the carcinogenesis of NSCLC and might be an early diagnostic biomarker for NSCLC detection.
- Subjects :
- Original Article
neoplasms
respiratory tract diseases
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.pmid..........c7803686f4df7ff912eae60720b329a2