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Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis culminating in enucleation

Authors :
Kaori Miyashima, Furuta
Shri Ram, Yadav
Satu, Lehesranta
Ilya, Belevich
Shunsuke, Miyashima
Jung-ok, Heo
Anne, Vatén
Ove, Lindgren
Bert, De Rybel
Gert, Van Isterdael
Panu, Somervuo
Raffael, Lichtenberger
Raquel, Rocha
Siripong, Thitamadee
Sari, Tähtiharju
Petri, Auvinen
Tom, Beeckman
Eija, Jokitalo
Ykä, Helariutta
Source :
Science (New York, N.Y.). 345(6199)
Publication Year :
2014

Abstract

Photoassimilates such as sugars are transported through phloem sieve element cells in plants. Adapted for effective transport, sieve elements develop as enucleated living cells. We used electron microscope imaging and three-dimensional reconstruction to follow sieve element morphogenesis in Arabidopsis. We show that sieve element differentiation involves enucleation, in which the nuclear contents are released and degraded in the cytoplasm at the same time as other organelles are rearranged and the cytosol is degraded. These cellular reorganizations are orchestrated by the genetically redundant NAC domain-containing transcription factors, NAC45 and NAC86 (NAC45/86). Among the NAC45/86 targets, we identified a family of genes required for enucleation that encode proteins with nuclease domains. Thus, sieve elements differentiate through a specialized autolysis mechanism.

Details

ISSN :
10959203
Volume :
345
Issue :
6199
Database :
OpenAIRE
Journal :
Science (New York, N.Y.)
Accession number :
edsair.pmid..........d5ba0b47ce7ae389fcd5f7c29181cb02