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Comparison of Commercial Enzyme-linked Immunosorbent Assays for Diagnosis of Contagious Agalactia Caused By

Authors :
Antonio, Sánchez
Antonio, Contreras
María L, Sánchez-Corral
Carmen, Martínez-Nista
Soledad, Collado
José L, Sáez
Olga, Minguez
Christian, de la Fe
Source :
Journal of veterinary research. 66(1)
Publication Year :
2021

Abstract

Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especiallyThis study was designed to appraise the performance of two commercial indirect ELISA tests usingAll three ELISA tests showed high validity scores (Youden's J: 72.9-84%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762-0.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93).The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting

Details

ISSN :
24507393
Volume :
66
Issue :
1
Database :
OpenAIRE
Journal :
Journal of veterinary research
Accession number :
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