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Human Liver Spheroids as a Model to Study Aetiology and Treatment of Hepatic Fibrosis
- Source :
- Cells, Cells, Vol 9, Iss 964, p 964 (2020), Volume 9, Issue 4
- Publication Year :
- 2020
-
Abstract
- Non-alcoholic fatty liver disease affects approximately one billion adults worldwide. Non-alcoholic steatohepatitis (NASH) is a progressive disease and underlies the advancement to liver fibrosis, cirrhosis, and hepatocellular carcinoma, for which there are no FDA-approved drug therapies. We developed a hetero-cellular spheroid system comprised of primary human hepatocytes (PHH) co-cultured with crude fractions of primary human liver non-parenchymal cells (NPC) from several matched or non-matched donors, to identify phenotypes with utility in investigating NASH pathogenesis and drug screening. Co-culture spheroids displayed stable expression of hepatocyte markers (albumin, CYP3A4) with the integration of stellate (vimentin, PDGFR&beta<br />), endothelial (vWF, PECAM1), and CD68-positive cells. Several co-culture spheroids developed a fibrotic phenotype either spontaneously, primarily observed in PNPLA3 mutant donors, or after challenge with free fatty acids (FFA), as determined by COL1A1 and &alpha<br />SMA expression. This phenotype, as well as TGF&beta<br />1 expression, was attenuated with an ALK5 inhibitor. Furthermore, CYP2E1, which has a strong pro-oxidant effect, was induced by NPCs and FFA. This system was used to evaluate the effects of anti-NASH drug candidates, which inhibited fibrillary deposition following 7 days of exposure. In conclusion, we suggest that this system is suitable for the evaluation of NASH pathogenesis and screening of anti-NASH drug candidates.
Details
- ISSN :
- 20734409
- Volume :
- 9
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cells
- Accession number :
- edsair.pmid.dedup....11516c07d94a55081993969e38e8e53a