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Parkin-mediated ubiquitination contributes to the constitutive turnover of mitochondrial fission factor (Mff)
- Source :
- PLoS ONE, Vol 14, Iss 5, p e0213116 (2019), PLoS ONE, Lee, L, Seager, R, Nakamura, Y, Wilkinson, K & Henley, J 2019, ' Parkin-mediated ubiquitination contributes to the constitutive turnover of mitochondrial fission factor (Mff) ', PLoS ONE, vol. 14, no. 5, e0213116 . https://doi.org/10.1371/journal.pone.0213116
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- The mitochondrial outer membrane protein Mitochondrial Fission Factor (Mff) plays a key role in both physiological and pathological fission. It is well established that at stressed or functionally impaired mitochondria, PINK1 recruits the ubiquitin ligase Parkin which ubiquitinates Mff and other mitochondrial outer membrane proteins to facilitate the removal of defective mitochondria and maintain the integrity of the mitochondrial network. Here we show that, in addition to this clearance pathway, Parkin also ubiquitinates Mff in a PINK1-dependent manner under non-stressed conditions to regulate constitutive Mff turnover. We further show that removing Parkin via shRNA-mediated knockdown does not completely prevent Mff ubiquitination under these conditions, indicating that at least one other ubiquitin ligase contributes to Mff proteostasis. These data suggest that that Parkin plays a role in physiological maintenance of mitochondrial membrane protein composition in unstressed cells through constitutive low-level activation.
- Subjects :
- Ubiquitin-Protein Ligases
Science
Cell Membranes
Cell Culture Techniques
Bioenergetics
Transfection
Research and Analysis Methods
Mitochondrial Dynamics
Biochemistry
Mitochondrial Proteins
Genetics
Humans
Small interfering RNAs
Post-Translational Modification
Phosphorylation
Molecular Biology Techniques
Non-coding RNA
Molecular Biology
Energy-Producing Organelles
Ubiquitination
Quantitative Analysis
Membrane Proteins
Biology and Life Sciences
Proteins
Cell Biology
Outer Membrane Proteins
Mitochondria
Gene regulation
nervous system diseases
Nucleic acids
HEK293 Cells
Research Design
Gene Knockdown Techniques
Proteolysis
RNA
Medicine
Gene expression
Cellular Structures and Organelles
Lysosomes
Protein Kinases
Signal Transduction
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.pmid.dedup....145142c1ee35f18db63f8973d47c715c