Back to Search
Start Over
N- and O-linked glycosylation site profiling of the human basic salivary proline-rich protein 3M
- Source :
- Journal of separation science. 39(10)
- Publication Year :
- 2015
-
Abstract
- In the present study, we show that the heterogeneous mixture of glycoforms of the basic salivary proline-rich protein 3M, encoded by PRB3-M locus, is a major component of the acidic soluble fraction of human whole saliva in the first years of life. Reversed-phase high-performance liquid chromatography with high-resolution electrospray ionization mass spectrometry analysis of the intact proteoforms before and after N-deglycosylation with Peptide-N-Glycosidase F and tandem mass spectrometry sequencing of peptides obtained after Endoproteinase GluC digestion allowed the structural characterization of the peptide backbone and identification of N- and O-glycosylation sites. The heterogeneous mixture of the proteoforms derives from the combination of 8 different neutral and sialylated glycans O-linked to Threonine 50, and 33 different glycans N-linked to Asparagine residues at positions 66, 87, 108, 129, 150, 171, 192, and 213.
Details
- ISSN :
- 16159314
- Volume :
- 39
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of separation science
- Accession number :
- edsair.pmid.dedup....1fb1dacb3cdb8abdc406125c749fca6c