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HIV-1-neutralizing antibody induced by simian adenovirus- and poxvirus MVA-vectored BG505 native-like envelope trimers
- Source :
- PLoS ONE, Vol 12, Iss 8, p e0181886 (2017), PLOS ONE, PLoS ONE, PLoS ONE, 12(8). Public Library of Science
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Rabbits and monkeys immunized with HIV type 1 (HIV-1) native-like BG505 SOSIP.664 (BG505s) glycoprotein trimers are known to induce antibodies that can neutralize the autologous tier-2 virus. Here, we assessed the induction of HIV-1 trimer binding and neutralizing antibody (nAb) titres when BG505s trimers were also delivered by non-replicating simian (chimpanzee) adenovirus and non-replicating poxvirus modified vaccinia virus Ankara (MVA) vaccine vectors. First, we showed that approximately two-thirds and one-third of the trimers secreted from the ChAdOx1.BG505s (C) and MVA.BG505s (M) vaccine-infected cells, respectively, were cleaved and in a native-like conformation. Rabbits were immunized intramuscularly with these vaccine vectors and in some cases boosted with ISCOMATRIX™-adjuvanted BG505s protein trimer (P), using CCC, MMM, PPP, CPP, MPP and CMP vaccine regimens. We found that the peak trimer-binding antibody and tier-1A and autologous tier-2 nAb responses induced by the CC, CM, PPP, CPP, MPP and CMP regimens were comparable, although only PPP induced autologous tier-2 nAbs in all the immunized animals. Three animals developed weak heterologous tier-2 nAbs. These results demonstrate that ChAdOx1 and MVA vectors are useful delivery modalities for not only T-cell, but also antibody vaccine development.
- Subjects :
- RNA viruses
Adenoviruses
Physiology
viruses
Disease Vectors
HIV Antibodies
Pathology and Laboratory Medicine
Biochemistry
Immunodeficiency Viruses
Immune Physiology
Medicine and Health Sciences
Vaccines, DNA
Enzyme-Linked Immunoassays
Mammals
AIDS Vaccines
Vaccines
Immune System Proteins
Vaccination
env Gene Products, Human Immunodeficiency Virus
Animal Models
Infectious Diseases
Experimental Organism Systems
Medical Microbiology
Viral Pathogens
Vertebrates
Viruses
Medicine
Rabbits
Pathogens
Viral Vectors
Research Article
Infectious Disease Control
Science
Immunology
Vaccinia virus
Research and Analysis Methods
Microbiology
Antibodies
Cell Line
Virology
Retroviruses
Animals
Humans
Antigens
Immunoassays
Microbial Pathogens
Lentivirus
Organisms
Biology and Life Sciences
Proteins
HIV
Viral Vaccines
Antibodies, Neutralizing
Species Interactions
HEK293 Cells
Amniotes
Immunologic Techniques
HIV-1
Adenoviruses, Simian
Protein Multimerization
DNA viruses
Viral Transmission and Infection
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.pmid.dedup....2f811463bb5a20af43b5fc73e9dcad9e